There are 247 medium confidence (MC) neoantigens bound by HLA-I molecules in dbPepNeo, each of which contains mutated amino acid. They were considered as HLA-Ⅰ binding neoantigens, the screening process relies on the combination of whole-exome sequencing and HLA peptidome mass spectrometry. The cancer type or cell lines contained are as follows: melanoma, non-small cell lung cancer, HCT 116 cell line.